Evaluation of HBs Ag, HCV and HIV Ag-Ab Assays using Bio-Rad Elite Microplate Analyzer

[Evaluation of HBs Ag, HCV and HIV Ag-Ab Assays using Bio-Rad Elite Microplate Analyzer.].

BACKGROUND In this study, we evaluated the performance of Elite microplate analyzer (Bio-Rad Laboratories, France) and the related assays (ULTRA line) for the detection of hepatitis B virus (HBV), hepatitis C virus (HCV) and human immunodeficiency virus (HIV). METHODS Seroconversion panels, HBsAg positive/HBsAg negative (n=99/n=1,422), anti-HCV positive/negative (n=97/n=1,670), and anti-HIV p...

Evaluation of the Abbott PRISM HIV Ag/Ab Combo Assay

Specimens from a single blood donor population as well as a single diagnostic population were tested on the Abbott PRISM HIV Ag/Ab Combo assay. All samples initially reactive were retested in duplicate. Repeatedly reactive specimens were tested in the NRL HIV-1/2 confirmatory algorithm to determine their true status. Specimens that were confirmed as HIV-positive were excluded from calculation o...

(HBs-Ag) and anti-HCV Antibody Among Health Care Workers of Canton Sarajevo (Bosnia and Herzegovina)

ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens

Periodic evaluation of the impact of viral diversity on diagnostic tests is critical to ensure current technologies are keeping pace with viral evolution. To determine whether HIV diversity impacts the ARCHITECT HIV Combo Ag/Ab (HIV Combo) or RealTime HIV-1 (RT) assays, a set of N = 199 HIV clinical specimens from Cameroon, Senegal, Saudi Arabia, and Thailand were sequenced and tested in both a...

Prevalence of HBc-Ab among HBs-Ag negative healthy blood donors in south of Iran

BACKGROUND The risk of infection with transfusion-transmitted viruses, such as Hepatitis B virus (HBV), has been reduced markedly. However, a zero-risk blood supply remains a popular goal. Anti-hepatitis B core antigen (anti-HBc) is considered to be an effective marker for occult HBV infection, and it has served a significant role in improving blood safety. The purpose of this study was to dete...